The lysate is then loaded onto the RNeasy silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. Ethanol is added to the lysate to provide ideal binding conditions. Samples are first lysed and then homogenized. RNeasy technology simplifies total RNA isolation (see table “Amount of starting material for RNeasy procedures”). Individual variations are low throughout the entire purification process TaqMan ® threshold-cycle values are easily achieved at the end of the process with a coefficient of variation (CV) less than 3% using the QuantiTect Probe RT-PCR Kit (see figure "Repeatability of fully automated RNA purification"). Sample sizes range from 10 to 5 x 10 5 cells (see figure "RT-PCR of RNA from as few as 100 cells"), and high-quality RNA can be purified from large numbers of samples (see figure "Reproducible yields of high-quality RNA"). The RNA is suitable for sensitive applications such as quantitative, real-time RT-PCR and microarray analysis (see figure "High-quality RNA for sensitive analysis of a low-copy transcript"). The RNeasy 96 system provides fast and reproducible total RNA purification for high-throughput gene expression profiling. Total RNA is easily purified with the RNeasy Maxi Kit from large amounts of starting material including animal or human cells, animal or human tissues, and yeast cells (see table “Total RNA yields obtained with RNeasy Kits”). Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA from a variety of samples"). The dedicated RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits"). A low score means a lower environmental impact (see figures "RNeasy Mini Kit ACT environmental impact factor label US, EU and UK"įor an eco-friendlier alternative to this kit, see our QIAwave RNA Mini Kit. Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. disposal of the packaging at the end of life.sustainable content within products and packaging materials.
My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. In partnership with My Green Lab, we've also assessed the environmental impact of the RNeasy Mini Kit (250). A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured-cell samples using silica-membrane RNeasy 96 plates.
Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica-membrane RNeasy spin columns or 96-well plates. RNeasy Kits are the Gold standard for total RNA isolation.
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